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DNA polymerases are enzymes that synthesize DNA molecules from nucleoside triphosphates. Products include various types of DNA polymerases, kits, buffers, and other reagents for DNA amplification.
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phi29 DNA Polymerase is the replicative polymerase from the Bacillus subtilis phage phi29. This polymerase has exceptional strand displacement and processive synthesis properties. The polymerase has an inherent 3' to 5' proofreading exonuclease activity.
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Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 53 polymerase activity and a 5 flap endonuclease activity. It is supplied with 10X ThermoPol Reaction Buffer, which contains a nonionic detergent to increase enzyme stability during longer incubations.
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Vent DNA Polymerase is a high-fidelity thermophilic DNA polymerase. The fidelity of Vent DNA Polymerase is 5-15-fold higher than that observed for Taq DNA Polymerase. This high fidelity derives in part from an integral 3' to 5' proofreading exonuclease activity in Vent DNA Polymerase. Greater than 90% of the polymerase activity remains following a 1 hour incubation at 95C.
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DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3' to 5' exonuclease activity, but has lost 5' to 3' exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.
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Therminator DNA Polymerase is a 9N DNA Polymerase variant with an enhanced ability to incorporate modified substrates such as dideoxynucleotides, ribonucleotides and acyclonucleotides.
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Takara LA Taq DNA polymerase paired with two special GC-optimized Buffers. This system is designed to amplify templates with up to 73% GC content, and up to 20 kb in length routinely (extension up to 48 kb is possible on some templates). 125 units
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Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Taq Polymerase includes 10x Ammonium Buffer and 10x Combination Buffer
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